Characterisation of Kenyan Isolates of Plasmodium Falciparum by Isoenzyme Electrophoresis and Drug Sensitivity.
Ofulla, Ayub v
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Twenty one Kenyan isolates of Plasmodium falciparum were characterised by enzyme electrophoresis and antimalarial drug sensitivity. For isoenzyme analysis, cellulose acetate and thin layer starch gel electrophoresis techniques were employed. P. falciparum isolates from malarious areas of Nyanza, Rift Valley and Coast provinces were cultivated in vitro and used in the isoenzyme study. The enzymes of uninfected red blood cells served as controls. The enzymes studied were: Glucose phosphate isomerase (GPI)(EC 5.3.19), 6-phosphogluconate dehydrogenase (6PGD)(EC 18.104.22.168), Lactate dehydrogenase (LDH)(EC 22.214.171.124), NADPdependent glutamate dehydrogenase (GDH)(EC 126.96.36.199), Adenosine deaminase (ADA)(EC 188.8.131.52) and Pepti~ase E (PEPE)(EC 3.4.11 or 13). The sensitivities of cultured isolates to seven antimalarial drugs (chloroquine, amodiaquine, quinine, quinidine, mefloquine, pyrimethamine and sulphadoxine) were examined by the in vitro radioisotopic methods of Desjardins et al. (1979) with minor modifications. Variant forms of the enzymes GPI and 6PGD were found in the Kenyan isolates of E. falciparum. GPI enzymes appeared as GPI-l and GPI-2 variants and 6PGD as 6PGD-l and 6PGD-2 variants. The level of enzyme polymorphism was greater in xvi GPI than in 6PGD. The isolates from Rift Valley province were found to possessa lower frequency of GPI-2 isoenzymes than isolates from Nyanza and Coast provinces. The other four enzymes (GDH, LDH, ADA and PEPE) appeared to be invariant in all the isolates. ADA-2 forms were found in all the isolates while the other three enzymes appeared as GDH-l, LDH-l isolates examined. Considerable variation was found in the antimalarial and PEPE-l types in all the drug response of the 21 parasite isolates. The Rift Valley province isolates showed significantly lower susceptibilities to most of the antimalarial drugs tested than the Coast and Nyanza province isolates. Coast Province isolates were found to be marginally more sensitive to the antimalarial drugs than the Nyanza province isol?tes.
University of Nairobi
SubjectCharacterisation of Kenyan Isolates of Plasmodium Falciparum by Isoenzyme Electrophoresis and Drug Sensitivity.
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