Enzymatic Hydrolysis of fish waste, characterization of Hydrolysate and Proximate analysis
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Date
2020Author
Okuku, Alice, S. O
Type
ThesisLanguage
enMetadata
Show full item recordAbstract
The fish industry generates a lot of waste which is attributable to the fact that there is an increased
demand for fish and that filleting of tilapia fish yields about 37% skin-off fillets. Enzymatic
hydrolysis used in this research project affords the prospect to apply rudimentary alkaline protease
obtained from extremophile bacteria in processing fish waste. The optimum working conditions
of the crude alkaline protease were established to be at 80⁰C, pH12 and a crude enzyme
concentration of 100%. The Amino acid composition was determined using HPLC while fatty acid
analysis using the FAME method using GC equipped with the FID. Proximate analysis was
determined in accordance with the standard test methods. The parameters evaluated included
moisture, crude protein, ash, carbohydrates and fats. The kinetics of the reaction showed that it
was zero order with respect to the concentration of the reactants. Glycine and alanine were
determined to be the peak amino acids present in the Fish Protein Hydrolysate, while histidine was
the least abundant. Fatty acid composition study disclosed that palmitic acid was most prominent
and the least abundant was lauric acid. Proximate analysis of the freeze-dried hydrolysate yielded
crude 6 protein, moisture, ash, crude fat, fibre and carbohydrates mean content of 54.2%,10.4 %,
21.8 %, 12.8 %, 0.6 % and 0.2 % respectively. The results demonstrate that the crude enzyme used
in this study can be used for fish waste hydrolysis to obtain useful value-added products and can
be used effectively in managing the fish waste disposal problem.
Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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