dc.contributor.author | Mohamed, Yehia S. | |
dc.contributor.author | Borthwick, Nicola J. | |
dc.contributor.author | Moyo, Nathifa | |
dc.contributor.author | Murakoshi, Hayato | |
dc.contributor.author | Akahoshi, Tomohiro | |
dc.contributor.author | Siliquini, Francesca | |
dc.contributor.author | Hannoun, Zara | |
dc.contributor.author | Crook, Alison | |
dc.contributor.author | Hayes, Peter | |
dc.contributor.author | Fast, Patricia E. | |
dc.contributor.author | Mutua, Gaudensia | |
dc.contributor.author | Jaoko, Walter | |
dc.contributor.author | Silva-Arrieta, Sandra | |
dc.contributor.author | Llano, Anuska | |
dc.contributor.author | Brander, Christian | |
dc.contributor.author | Takiguchi, Masafumi | |
dc.contributor.author | Hanke, Tomáš | |
dc.date.accessioned | 2020-11-20T08:29:56Z | |
dc.date.available | 2020-11-20T08:29:56Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Mohamed, Y.S.; Borthwick, N.J.; Moyo, N.; Murakoshi, H.; Akahoshi, T.; Siliquini, F.; Hannoun, Z.; Crook, A.; Hayes, P.; Fast, P.E.; Mutua, G.; Jaoko, W.; Silva-Arrieta, S.; Llano, A.; Brander, C.; Takiguchi, M.; Hanke, T. Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya. Vaccines 2020, 8, 260. | en_US |
dc.identifier.uri | https://www.mdpi.com/2076-393X/8/2/260#cite | |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/153461 | |
dc.description.abstract | Sub-Saharan Africa carries the biggest burden of the human immunodeficiency virus type 1 (HIV-1)/AIDS epidemic and is in an urgent need of an effective vaccine. CD8+ T cells are an important component of the host immune response to HIV-1 and may need to be harnessed if a vaccine is to be effective. CD8+ T cells recognize human leukocyte antigen (HLA)-associated viral epitopes and the HLA alleles vary significantly among different ethnic groups. It follows that definition of HIV-1-derived peptides recognized by CD8+ T cells in the geographically relevant regions will critically guide vaccine development. Here, we study fine details of CD8+ T-cell responses elicited in HIV-1/2-uninfected individuals in Nairobi, Kenya, who received a candidate vaccine delivering conserved regions of HIV-1 proteins called HIVconsv. Using 10-day cell lines established by in vitro peptide restimulation of cryopreserved PBMC and stably HLA-transfected 721.221/C1R cell lines, we confirm experimentally many already defined epitopes, for a number of epitopes we define the restricting HLA molecule(s) and describe four novel HLA-epitope pairs. We also identify specific dominance patterns, a promiscuous T-cell epitope and a rescue of suboptimal T-cell epitope induction in vivo by its functional variant, which all together inform vaccine design. View Full-Text | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.subject | HIV vaccine; HIVconsv; conserved regions; CD8 epitopes; HLA class I epitopes; T cell vaccine; African HLA | en_US |
dc.title | Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya | en_US |
dc.type | Article | en_US |