dc.contributor.author | Mabuka, Jennifer | |
dc.contributor.author | Nduati, Ruth | |
dc.contributor.author | Odem-Davis, Katherine | |
dc.contributor.author | Peterson, Dylan | |
dc.contributor.author | Overbaugh, Julie | |
dc.date.accessioned | 2020-11-27T09:48:23Z | |
dc.date.available | 2020-11-27T09:48:23Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Mabuka J, Nduati R, Odem-Davis K, Peterson D, Overbaugh J (2012) HIV-Specific Antibodies Capable of ADCC Are Common in Breastmilk and Are Associated with Reduced Risk of Transmission in Women with High Viral Loads. PLoS Pathog 8(6): e1002739. doi:10.1371/journal.ppat.1002739 | en_US |
dc.identifier.uri | http://erepository.uonbi.ac.ke/handle/11295/153525 | |
dc.description.abstract | There are limited data describing the functional characteristics of HIV-1 specific antibodies in breast milk (BM) and their role
in breastfeeding transmission. The ability of BM antibodies to bind HIV-1 envelope, neutralize heterologous and autologous
viruses and direct antibody-dependent cell cytotoxicity (ADCC) were analyzed in BM and plasma obtained soon after
delivery from 10 non-transmitting and 9 transmitting women with high systemic viral loads and plasma neutralizing
antibodies (NAbs). Because subtype A is the dominant subtype in this cohort, a subtype A envelope variant that was
sensitive to plasma NAbs was used to assess the different antibody activities. We found that NAbs against the subtype A
heterologous virus and/or the woman’s autologous viruses were rare in IgG and IgA purified from breast milk supernatant
(BMS) – only 4 of 19 women had any detectable NAb activity against either virus. Detected NAbs were of low potency
(median IC50 value of 10 versus 647 for the corresponding plasma) and were not associated with infant infection (p = 0.58).
The low NAb activity in BMS versus plasma was reflected in binding antibody levels: HIV-1 envelope specific IgG titers were
2.2 log10 lower (compared to 0.59 log10 lower for IgA) in BMS versus plasma. In contrast, antibodies capable of ADCC were
common and could be detected in the BMS from all 19 women. BMS envelope-specific IgG titers were associated with both
detection of IgG NAbs (p = 0.0001)and BMS ADCC activity (p = 0.014). Importantly, BMS ADCC capacity was inversely
associated with infant infection risk (p = 0.039). Our findings indicate that BMS has low levels of envelope specific IgG and
IgA with limited neutralizing activity. However, this small study of women with high plasma viral loads suggests that
breastmilk ADCC activity is a correlate of transmission that may impact infant infection risk. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
dc.title | HIV-Specific Antibodies Capable of ADCC Are Common in Breastmilk and Are Associated with Reduced Risk of Transmission in Women with High Viral Loads | en_US |
dc.type | Article | en_US |