In vitro Antiplasmodial and Cytotoxic activity of Three Medicinal Plants used Traditionally for Treatment of Malaria
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Date
2020Author
Omole, Ruth A
moshi, Mainen J
Ilias, Muhammad
Larry, Walker
Malebo, Hamisi M.
Omosa, Leonida K
Midiwo, Jacob O.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Introduction: Reports of emergence of Artemisinin Combination Therapies (ACTs) resistant malaria parasites in Greater Mekong region and Equatorial Guinea, is a strong reason necessitating increased efforts to discover
new antimalarial compounds with novel mechanisms of action. Plants have
potential to yield new antiplasmodial compounds. This study investigated
the safety and efficacy of three plants; Bersama abyssinica Fresen, Rubus
keniensis Standl and Hypoestes verticillaris (L.f.) Sol. ex Roem. and Schult
that are used by the Ogiek community of Kenya for treatment of malaria.
Methodology: The crude extracts were tested for in vitro antimalarial activity using Plasmodium falciparum strains W2 (chloroquine resistant) and D6
(chloroquine sensitive). Safety evaluation was done using monkey kidney
Vero cells and the brine shrimp lethality test. Results: Dichloromethane:
methanol (1:1) and 5% aqueous methanol extracts of the three plants exhibited in vitro antiplasmodial activity against the W2 and D6 Plasmodium
falciparum strains with IC50= 12.11–19.18 µg/mL, 5.46-7.04 µg/mL and 9.82
– 34.52 µg/mL, respectively. H. verticillaris extracts were the most active
against the two Plasmodium falciparum strains. The dichloromethane:
methanol extracts of the three plants exhibited lower toxicity on monkey
kidney Vero cells relative to antiplasmodial activity as compared to the
5% aqueous methanol extracts. The mean Vero cells: parasite selectivity
index of the dichloromethane: methanol extracts was (4.8), B. abyssinica
(3.75) and R. keniensis (1.9), while for the 5% aqueous methanol extracts
they were H. verticillaris (1.0), B. abyssinica (1.95) and R. keniensis (1.75).
A similar toxicity profile was exhibited by brine shrimp lethality results.
Conclusion: The results support the use of the three plants for the treatment of malaria. Therefore, they have potential to yield safe and effective
compounds targeting P. falciparum malaria.
URI
https://profiles.uonbi.ac.ke/lkerubo/files/omole_et_al_2020.pdfhttps://profiles.uonbi.ac.ke/lkerubo/publications/vitro-antiplasmodial-and-cytotoxic-activity-three-medicinal-plants-used-traditi
http://erepository.uonbi.ac.ke/handle/11295/155469
Citation
Omole RA, Moshi MJ, Ilias M, Larry W, Malebo HM, Omosa LK, Midiwo JO. "In vitro Antiplasmodial and Cytotoxic activity of Three Medicinal Plants used Traditionally for Treatment of Malaria." Investigational Medicinal Chemistry & Pharmacology. 2020;10(1):2-6.Publisher
University of Nairobi
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Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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