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dc.contributor.authorOsoti, Victor
dc.contributor.authorAkinyi, Mercy
dc.contributor.authorWamae, Kevin
dc.contributor.authorKimenyi, Kelvin M
dc.contributor.authorLaurent, Zaydah de
dc.contributor.authorNdwiga, Leonard
dc.contributor.authorGichuki, Paul
dc.contributor.authorOkoyo, Collins
dc.contributor.authorKepha, Stella
dc.contributor.authorMwandawiro, Charles
dc.contributor.authorKandie, Regina
dc.contributor.authorBejon, Philip
dc.contributor.authorSnow, Robert W
dc.contributor.authorOchola-Oyier, Lynette I
dc.date.accessioned2022-07-05T09:40:00Z
dc.date.available2022-07-05T09:40:00Z
dc.date.issued2022-04
dc.identifier.citationOsoti V, Akinyi M, Wamae K, Kimenyi KM, de Laurent Z, Ndwiga L, Gichuki P, Okoyo C, Kepha S, Mwandawiro C, Kandie R, Bejon P, Snow RW, Ochola-Oyier LI. Targeted Amplicon Deep Sequencing for Monitoring Antimalarial Resistance Markers in Western Kenya. Antimicrob Agents Chemother. 2022 Apr 19;66(4):e0194521. doi: 10.1128/aac.01945-21. Epub 2022 Mar 10. PMID: 35266823; PMCID: PMC9017353.en_US
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/35266823/
dc.identifier.urihttp://erepository.uonbi.ac.ke/handle/11295/161220
dc.description.abstractMolecular surveillance of Plasmodium falciparum parasites is important to track emerging and new mutations and trends in established mutations and should serve as an early warning system for antimalarial resistance. Dried blood spots were obtained from a Plasmodium falciparum malaria survey in school children conducted across eight counties in western Kenya in 2019. Real-time PCR identified 500 P. falciparum-positive samples that were amplified at five drug resistance loci for targeted amplicon deep sequencing (TADS). The absence of important kelch 13 mutations was similar to previous findings in Kenya pre-2019, and low-frequency mutations were observed in codons 569 and 578. The chloroquine resistance transporter gene codons 76 and 145 were wild type, indicating that the parasites were chloroquine and piperaquine sensitive, respectively. The multidrug resistance gene 1 haplotypes based on codons 86, 184, and 199 were predominantly present in mixed infections with haplotypes NYT and NFT, driven by the absence of chloroquine pressure and the use of lumefantrine, respectively. The sulfadoxine-pyrimethamine resistance profile was a "superresistant" combination of triple mutations in both Pfdhfr (51I 59R 108N) and Pfdhps (436H 437G 540E), rendering sulfadoxine-pyrimethamine ineffective. TADS highlighted the low-frequency variants, allowing the early identification of new mutations, Pfmdr1 codon 199S and Pfdhfr codon 85I and emerging 164L mutations. The added value of TADS is its accuracy in identifying mixed-genotype infections and for high-throughput monitoring of antimalarial resistance markers.en_US
dc.language.isoenen_US
dc.publisherUniversity of Nairobien_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectKenya; Plasmodium falciparum; antimalarial agents; deep sequencing; drug resistance.en_US
dc.titleTargeted Amplicon Deep Sequencing for Monitoring Antimalarial Resistance Markers in Western Kenyaen_US
dc.typeArticleen_US


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