Mechanism of Action of Endophytic Fungi Hypocrea Lixii and Beauveria Bassiana in Phaseolus Vulgaris as Biopesticides Against Pea Leafminer and Fall Armyworm
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Date
2021-09Author
Chebet, Olivia N
Omosa, Leonidah K
Subramanian, Sevgan
Nchiozem-Ngnitedem, Vaderament-A
Mmari, John O
Akutse, Komivi S
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Endophytic fungal isolates Hypocrea lixii F3ST1 and Beauveria bassiana G1LU3 were evalu-
ated for their potential to endophytically colonize and induce active compounds in Phaseolus vulgaris,
as a defense mechanism against pea leafminer (Liriomyza huidobrensis) and fall armyworm (Spodoptera
frugiperda). Endophytic colonization was achieved through seed inoculation with the volatile emis-
sions from P. vulgaris plants being analyzed using GC-MS. The crude extracts of P. vulgaris obtained
using methanol and dichloromethane were assayed against leafminer and fall armyworm larvae
using leaf dipping and topical application, respectively. The two isolates successfully colonized the
entire host plant (roots, stems, and leaves) with significant variation (p < 0.001) between fungal iso-
lates and the controls. The results showed qualitative differences in the volatile profiles between the
control plants, endophytically colonized and insect-damaged plants attributed to fungal inoculation
and leafminer damage. The crude methanol extracts significantly reduced the percentage pupation
of 2nd instar leafminer larvae (p < 0.001) and adult-flies emergence (p < 0.05). The survival of the 1st
instar fall armyworm larvae was also significantly reduced (p < 0.001) compared to the controls. This
study demonstrated the high potential of endophytic fungi H. lixii and B. bassiana in inducing mainly
specific defense compounds in the common bean P. vulgaris that can be used against pea leafminer
and fall armyworm.
Publisher
University of Nairobi
Rights
Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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