dc.contributor.author | Kaul, R. | |
dc.contributor.author | Thottingal, P. | |
dc.contributor.author | Kimani, J. | |
dc.contributor.author | Kiama, P. | |
dc.contributor.author | Waigwa, C. W. | |
dc.contributor.author | Bwayo, JJ | |
dc.contributor.author | Plummer, F. A. | |
dc.contributor.author | Rowland-Jones, S. L. | |
dc.date.accessioned | 2013-04-16T12:53:53Z | |
dc.date.available | 2013-04-16T12:53:53Z | |
dc.date.issued | 2003-05 | |
dc.identifier.citation | AIDS | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/12819514 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16161 | |
dc.description.abstract | BACKGROUND:
CD8 T lymphocytes are important in HIV-1 control and mediate virus-specific immunity in the blood and genital tract. The induction and monitoring of mucosal CD8 cell responses will be an important component of HIV-1 vaccine trials, but information regarding the frequency, phenotype and function of genital tract CD8 cell responses is lacking.
METHODS:
Simultaneous blood and cervical cytobrush samples were obtained from 16 HIV-1-infected Kenyan sex workers. Epitope-specific CD8 T lymphocyte frequencies in the blood and genital tract were analysed after short-term peptide incubation and intracellular cytokine staining for interferon-gamma (IFN gamma).
RESULTS:
Cervical sampling resulted in adequate cell numbers for analysis in 10/16 women. Background IFN gamma production was higher in CD3+/CD8+ lymphocytes from the genital tract than from blood (0.48% versus 0.1%; P < 0.01). Responses to staphylococcal enterotoxin B were detected in cervical CD8 lymphocytes from 10/10 women, at a similar frequency to blood (16.7% in cervix and 13.3% in blood; P = 0.4). HIV-1-specific responses were detected the cervix of 8/10 women, with a trend to higher response frequencies in the genital tract than blood (2.1% versus 0.8%; P = 0.09). Co-expression of integrin CD103 (alpha E beta 7), a mucosal marker, was used to confirm the mucosal origin of cervical responses.
CONCLUSIONS:
Cytobrush sampling and intracellular cytokine staining is well suited to the analysis of cervical CD8 cell responses. The frequency of functional virus-specific CD3+/CD8+ T cells is similar in the genital tract and blood of HIV-1-infected women. The role of genital tract CD8 cell responses in HIV-1 control warrants further investigation. | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Vol. 23;17(8):1139-44. (2003); | |
dc.title | Quantitative ex vivo analysis of functional virus-specific CD8 T lymphocytes in the blood and genital tract of HIV-infected women | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya | en |