dc.contributor.author | Hardie, RA | |
dc.contributor.author | Luo, M | |
dc.contributor.author | Bruneau B | |
dc.contributor.author | Knight, E | |
dc.contributor.author | Nagelkerke, NJ | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Wachihi, C | |
dc.contributor.author | Ngugi, Elizabeth N | |
dc.contributor.author | Plummer, FA | |
dc.date.accessioned | 2013-04-17T05:45:23Z | |
dc.date.available | 2013-04-17T05:45:23Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | AIDS. 2008 Apr 23;22(7):807-16. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/18427198 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16175 | |
dc.description.abstract | Objectives: To determine the association of DQ antigens with resistance and susceptibility
to HIV-1.
Design: Despite repeated exposure to HIV-1, a subset of women in the Pumwani Sex
Worker cohort established in Nairobi, Kenya in 1985 have remained HIV-1 negative for
at least 3 years and are classified as resistant. Differential susceptibility to HIV-1
infection is associated with HIV-1 specific CD4þ and CD8þ T cell responses. As human
leukocyte antigen-DQ antigens present viral peptides to CD4þ cells, we genotyped
human leukocyte antigen -DQ alleles for 978 women enrolled in the cohort and
performed cross-sectional and longitudinal analyses to identify associations of human
leukocyte antigen -DQ with resistance/susceptibility to HIV-1.
Methods: DQA1 and DQB1 were genotyped using taxonomy-based sequence
analysis. SPSS 13.0 was used to determine associations of DQ alleles/haplotypes with
HIV-1 resistance, susceptibility, and seroconversion rates.
Results: Several DQB1 alleles and DQ haplotypes were associated with resistance to
HIV-1 infection. These included DQB1 050301 (P¼0.055, Odds Ratio¼12.77, 95%
Confidence Interval¼1.44–112), DQB1 0603 and DQB1 0609 (P¼0.037, Odds
Ratio¼3.25, 95%Confidence Interval¼1.12–9.47), andDQA1 010201–DQB1 0603
0603 (P¼0.044, Odds Ratio¼17.33, 95% Confidence Interval¼1.79–168). Conversely,
DQB1 0602 (P¼0.048,Odds Ratio¼0.68, 95%Confidence Interval¼0.44–1.05)
and DQA1 010201–DQB1 0602 (P¼0.039, Odds Ratio¼0.64, 95% Confidence
Interval¼0.41–1.03) were overrepresented in the HIV-1 infected population.
DQA1 0504-DQB1 0201, DQA1 010201–DQB1 0201, DQA1 0402-DQB1 0402
and DQA1 0402-DQB1 030101 genotypes were only found in HIV-1 positive subjects
(Odds Ratio¼0.30-0.31, 95% Confidence Interval¼0.03–3.70), and these women
seroconverted rapidly. The associations of these DQ alleles and haplotypes with resistance and susceptibility to HIV-1 were independent of the previously reported human
leukocyte antigen-DRB 01, human leukocyte antigen A2/6802, and human leukocyte
antigen-A 2301.
Conclusion: The associations ofDQalleles and haplotypes with resistance and susceptibility to HIV-1 emphasize the importance of human leukocyte antigen-DQ and CD4 in anti-HIV-1 immunity. | en |
dc.language.iso | en | en |
dc.subject | Disease association | en |
dc.subject | Disease resistance | en |
dc.subject | DNA sequence analysis, HIV-1 | en |
dc.subject | Human leukocyte antigen-DQA1 | en |
dc.subject | Human leukocyte antigen-DQB1 | en |
dc.subject | Sex workers | en |
dc.title | Human leukocyte antigen-DQ alleles and haplotypes and their associations with resistance and susceptibility to HIV-1 infection | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada | en |
local.publisher | Department of Community Health, University of Nairobi, | en |
local.publisher | Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada, | en |
local.publisher | Department of Community Medicine, United Arab Emirates University, Al-Ain, United Arab Emirates | en |
local.publisher | Medical Microbiology, Room 507, Basic Medical Sciences Building, 730 William Avenue, | en |