Phenotypic characterization of phage vB_vcM_Kuja
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Date
2023Author
Maina, Alice N
Mwaura, Francis B
Wagacha, John M
Jumba, Miriam
Aziz, Ramy K
Nour, Hanzada T
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Bacteriophage therapy targeting the increasingly resistant Vibrio cholerae is highly needed. Hence, studying the phenotypic behavior of potential phages under different conditions is a prerequisite to delivering the phage in an active infective form. The objective of this study was to characterize phage VP4 (vB_vcM_Kuja), an environmental vibriophage isolated from River Kuja in Migori County, Kenya in 2015. The phenotypic characteristics of the phage were determined using a one-step growth curve, restriction digestion profile, pH, and temperature stability tests. The results revealed that the phage is stable through a wide range of temperatures (20-50°C) and maintains its plaque-forming ability at pH ranging from 6 to 12. The one-step growth curve showed a latent period falling between 40 and 60 min, while burst size ranged from 23 to 30 plaque-forming units/10 µl at the same host strain. The restriction digestion pattern using EcoRI, SalI, HindIII, and XhoI enzymes showed that HindIII could cut the phage genome. The phage DNA could not be restricted by the other three enzymes. The findings of this study can be used in future studies to determine phage-host interactions.
Citation
Maina AN, Mwaura FB, Wagacha JM, Jumba M, Aziz RK, Nour El-Din HT. Phenotypic characterization of phage vB_vcM_Kuja. J Basic Microbiol. 2023 Jan 20. doi: 10.1002/jobm.202200635. Epub ahead of print. PMID: 36670071.Publisher
University of Nairobi
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Attribution-NonCommercial-NoDerivs 3.0 United StatesUsage Rights
http://creativecommons.org/licenses/by-nc-nd/3.0/us/Collections
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