An ARID family protein binds to the African swine fever virus encoded ubiquitin conjugating enzyme, UBCv1.
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Date
2000-04Author
Miskin, J. E.
Dixon, L. K.
Bulimo, Wallace D.
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The NH(2)-terminal end of a protein, named SMCp, which contains an ARID (A/T rich interaction domain) DNA binding domain and is similar to the mammalian SMCY/SMCX proteins and retinoblastoma binding protein 2, was shown to bind the African swine fever virus encoded ubiquitin conjugating enzyme (UBCv1) using the yeast two hybrid system and in in vitro binding assays. Antisera raised against the SMCp protein were used to show that the protein is present in the cell nucleus. Immunofluorescence showed that although UBCv1 is present in the nucleus in most cells, in some cells it is in the cytoplasm, suggesting that it shuttles between the nucleus and cytoplasm. The interaction and co-localisation of UBCv1 with SMCp suggest that SMCp may be a substrate in vivo for the enzyme
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http://www.sciencedirect.com/science/article/pii/S0014579300013521http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16492
Citation
FEBS Lett.Publisher
Department of Medicine. College of Health Sciences. University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10378]