dc.contributor.author | Mukuria, J C | |
dc.contributor.author | Naiki, M | |
dc.contributor.author | Hashimoto, M | |
dc.contributor.author | Kato, S | |
dc.date.accessioned | 2013-04-22T14:52:06Z | |
dc.date.available | 2013-04-22T14:52:06Z | |
dc.date.issued | 1986-02-12 | |
dc.identifier.citation | Journal of immunological methods, 86(2): 179-185 | en |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/0022175986904503 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16532 | |
dc.description | Journal article | en |
dc.description.abstract | A specific, relatively sensitive, quantitative and standardized enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of heterophile Hanganutziu and Deicher (HD) antibodies which are occasionally elevated in pathologic human sera. The HD antigen-active molecule used for the assay was a ganglioside (N-glycolylneuraminyllactosylceramide, abbreviated as NeuGc-LacCer) previously purified from horse erythrocyte membranes. The test used antigen-coated plastic microtiter plates and anti-human immunoglobulin G (IgG, Fab fragment) conjugated with alkaline phosphatase. Fifty-four normal human sera gave ELISA values ranging from −2 to 2%. Random sera from hospitalized patients were first screened by the horse erythrocyte hemagglutination (HA) test, whereby 5.7% (76 cases) gave abnormal HA titers of 128–4096 compared to titers in normal sera equal to or less than 64. Ninety-seven % of the patients' sera gave abnormal ELISA values (3–200%). They were classified into 3 groups: cancer (42 cases), infection (10 cases), and others (24 cases). The potential value of this ELISA method is discussed. | en |
dc.language.iso | en | en |
dc.subject | ELISA; | en |
dc.subject | Hanganutziu and Deicher antibodies | en |
dc.subject | Hemagglutination | en |
dc.subject | Heterophile | en |
dc.subject | N-glycolylneuraminic acid | en |
dc.title | A specific enzyme-linked immunosorbent assay (ELISA) procedure for detection of heterophile Hanganutziu and Deicher (HD) antibodies | en |
dc.type | Article | en |
local.publisher | College of Health Sciences, University of Nairobi | en |