dc.contributor.author | Zeh, C, | |
dc.contributor.author | Oyaro, B | |
dc.contributor.author | Vandenhoudt, H | |
dc.contributor.author | Amornkul, P | |
dc.contributor.author | Kasembeli, A, | |
dc.contributor.author | Bondo, P | |
dc.contributor.author | Mwaengo, D | |
dc.contributor.author | Thomas, T.K, | |
dc.contributor.author | Hart, C | |
dc.contributor.author | Laserson, K.F | |
dc.contributor.author | Ondoa, P | |
dc.contributor.author | Nkengasong, J.N | |
dc.date.accessioned | 2013-04-25T06:15:27Z | |
dc.date.available | 2013-04-25T06:15:27Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | J Virol Methods. 2011 Sep;176(1-2):24-31 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/16647 | |
dc.description.abstract | Performances of serological parallel and serial testing algorithms were analyzed using a combination of three ELISA and three rapid tests for the confirmation of HIV infection. Each was assessed individually for their sensitivity and specificity on a blinded panel of 769 retrospective sera of known HIV status. Western blot was used as a confirmatory assay for discordant results. Subsequently, one parallel and one serial testing algorithm were assessed on a new panel of 912 HIV-positive and negative samples. Individual evaluation of the ELISAs and rapid tests indicated a sensitivity of 100% for all assays except Uni-Gold with 99.7%. The specificities ranged from 99.1% to 99.4% for rapid assays and from 97.5% to 99.1% for ELISAs. A parallel and serial testing algorithms using Enzygnost and Vironostika, and Determine followed by Uni-Gold respectively, showed 100% sensitivity and specificity. The cost for testing 912 samples was US$4.74 and US$ 1.9 per sample in parallel and serial testing respectively. Parallel or serial testing algorithm yielded a sensitivity and specificity of 100%. This alternative algorithm is reliable and reduces the occurrence of both false negatives and positives. The serial testing algorithm was more cost effective for diagnosing HIV infections in this population. | en |
dc.language.iso | en | en |
dc.title | Performance of six commercial enzyme immunoassays and two alternative HIV-testing algorithms for the diagnosis of HIV-1 infection in Kisumu, Western Kenya. | en |
dc.type | Article | en |