dc.contributor.author | Mujugira, Andrew | |
dc.contributor.author | Morrow, Rhoda Ashley | |
dc.contributor.author | Celum, Connie | |
dc.contributor.author | Mugo, Nelly | |
dc.contributor.author | Vwalika, Bellington | |
dc.contributor.author | Baeten, Jared M | |
dc.date.accessioned | 2013-05-12T09:18:08Z | |
dc.date.available | 2013-05-12T09:18:08Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Sex Transm Infect. 2011 April; 87(3): 238–241. | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/22355 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/21307152 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066402/ | |
dc.description | Full text | en |
dc.description.abstract | Objective
To compare the performance of the Focus HerpeSelect-2 enzyme immunoassay (EIA) to the gold standard HSV-2 Western blot, among HIV-1 uninfected men and women in East and Southern Africa.
Methods
3399 HIV-1 uninfected women and men from 7 countries in East and Southern Africa were tested for HSV-2 antibody using Focus HerpeSelect-2 EIA. The performance of the HerpesSelect-2 EIA was compared with the gold standard HSV-2 specific Western blot.
Results
Two-thirds (2294/3399) of participants were male and two-thirds (2242/3399) were from East Africa. By Western blot testing, HSV-2 prevalence was 68%, 59% in men and 85% in women. At the manufacturer’s recommended cut-off value of greater than 1.1, the HerpeSelect-2 EIA had a sensitivity of 98.3% and specificity 80.3%. Receiver operating characteristic (ROC) plot analysis indicated that the optimum cut-off was 2.1 or greater with sensitivity 93.9% and specificity 90.5%. Diagnostic accuracy was modestly higher for Southern Africa (AUC=0.979, 95% CI: 0.970-0.988) compared with East Africa (AUC=0.954, 95% CI: 0.942-0.965; p<0.001 for Southern vs. East Africa).
Conclusions
The Focus HerpeSelect-2 EIA has acceptable diagnostic accuracy for determination of HSV-2 serostatus in African HIV-1 uninfected adults. An assay cut-off value of 2.1 or greater results in approximately 90% sensitivity and specificity, against a gold standard HSV-2 Western blot. Diagnostic accuracy differed slightly by geographical region. | en |
dc.language.iso | en | en |
dc.subject | HIV-1 | en |
dc.subject | HSV-2 | en |
dc.title | Performance of the Focus HerpeSelect-2 EIA for the detection of herpes simplex virus type 2 antibodies in seven African countries | en |
dc.type | Article | en |
local.publisher | Department of Obstetrics & Gynecology | en |