dc.contributor.author | Bulimo, Wallace D | |
dc.contributor.author | Mukunzi, Silvanos | |
dc.contributor.author | Achilla, Rachel | |
dc.contributor.author | Opot, Benjamin H. | |
dc.contributor.author | Osuna, Finley | |
dc.contributor.author | Majanja, Janet | |
dc.contributor.author | Wadegu, Meshack | |
dc.contributor.author | Wurapa, Eyako K | |
dc.date.accessioned | 2013-05-30T13:45:17Z | |
dc.date.available | 2013-05-30T13:45:17Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Were the WHO-recommended Human Influenza Vaccine Formulations Appropriate for Kenya During the 2010-2011 Season? Inferences from the HA1 Gene Analysis, Bulimo, Wallace D., Mukunzi Silvanos, Achilla Rachel, Opot Benjamin H., Osuna Finley, Majanja Janet, Wadegu Meshack, and Wurapa Eyako K. , African Journal of Pharmacology and Therapeutics, September 20012, Volume 1, Number 2, p.46-54, (2012) | en |
dc.identifier.uri | http://profiles.uonbi.ac.ke/wallacebulimo/publications/were-who-recommended-human-influenza-vaccine-formulations-appropriate-k-0 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/27811 | |
dc.description.abstract | Background: The knowledge of evolutionary patterns of the HA gene of the influenza virus is important in vaccine strain selection. Objective: Genetic analysis of HA1 of influenza viruses isolated in Kenya during the 2010-2011 season with reference to WHO vaccine strains. Methods: A total of twenty seven (27) influenza A (H1N1) pdm09, Nineteen (19) influenza A (H3N2) and Sixteen (16)influenza B virus isolates were analyzed. A partial HA1 gene was amplified by RT-PCR and sequenced. Results: Phylogenetic analyses revealed that influenza B viruses were closely related to B/Brisbane/60/2008 vaccine strain while A (H1N1) pdm09 viruses were genetic variants of A/California/07/2009. The Kenyan A (H1N1) pdm09isolates had P83S, D97N, S185T, I321V and E374K amino acid substitutions. Influenza A/H3N2 isolates showed K62E,T212A and S214I simultaneous amino acid substitutions when compared to A/Perth/10/2009. The K62E changeoccurred at antigenic site E. Majority of the Kenyan H3N2 isolates further had S45N and K144N amino acidsubstitutions at sites C and A respectively, which introduced N-glycosylation motifs absent in the vaccine strain.Conclusion: The study showed that although the WHO 2010 vaccine strains recommendations for the southern-hemisphere matched with influenza viruses which circulated in Kenya during the 2010-2011 season, the viruses had evolved genetically from the vaccine strains | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi. | en |
dc.title | Were the WHO-recommended Human Influenza Vaccine Formulations Appropriate for Kenya During the 2010-2011 Season? Inferences from the HA1 Gene Analysis | en |
dc.type | Presentation | en |
local.publisher | College of Health Sciences, University of Nairobi, Kenya | en |