Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes
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Date
2004Author
Beattiea, Tara
Kaulb, Rupert
Rostrona, Tim
Donga, Tao
Easterbrook, Philippa
Jaoko Walter G.
Kimani, Joshua
Plummer, Francis
McMichael, Andrew
Rowland-Jones, Sarah
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The IFN-ª enzyme-linked immunospot (ELISpot)
assay is often used to map HIV-specific
CD8 T-cell responses. We compared overlapping
15-mer pools with optimized CD8 epitopes to
screen ELISpot responses in HIV-infected individuals.
The 15-mer pools detected responses to
previously undefined epitopes, but often missed
low-level responses to predefined epitopes, particularly
when the epitope was central in the
15-mer, rather than at the N-terminus or Cterminus.
These factors should be considered in
the monitoring of HIV vaccine trials.
URI
http://www.ncbi.nlm.nih.gov/pubmed/15238779http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/28714
Citation
Research Letters AIDS 2004, 18:1595–1606Publisher
Univesity of Nairobi Department of Medicine