dc.contributor.author | Beattiea, Tara | |
dc.contributor.author | Kaulb, Rupert | |
dc.contributor.author | Rostrona, Tim | |
dc.contributor.author | Donga, Tao | |
dc.contributor.author | Easterbrook, Philippa | |
dc.contributor.author | Jaoko Walter G. | |
dc.contributor.author | Kimani, Joshua | |
dc.contributor.author | Plummer, Francis | |
dc.contributor.author | McMichael, Andrew | |
dc.contributor.author | Rowland-Jones, Sarah | |
dc.date.accessioned | 2013-06-03T11:37:21Z | |
dc.date.available | 2013-06-03T11:37:21Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Research Letters AIDS 2004, 18:1595–1606 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/15238779 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/28714 | |
dc.description.abstract | The IFN-ª enzyme-linked immunospot (ELISpot)
assay is often used to map HIV-specific
CD8 T-cell responses. We compared overlapping
15-mer pools with optimized CD8 epitopes to
screen ELISpot responses in HIV-infected individuals.
The 15-mer pools detected responses to
previously undefined epitopes, but often missed
low-level responses to predefined epitopes, particularly
when the epitope was central in the
15-mer, rather than at the N-terminus or Cterminus.
These factors should be considered in
the monitoring of HIV vaccine trials. | en |
dc.language.iso | en | en |
dc.publisher | Univesity of Nairobi | en |
dc.title | Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes | en |
dc.type | Article | en |
local.publisher | Department of Medicine | en |