A novel pseudohomogeneous radioimmunoassay for the measurement of plasma testosterone.
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Date
1983Author
Makawiti, D W
Barnard, G J
Matson, C M
Collins, W P
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A description is given of a novel liquid phase immunoassay for the measurement of testosterone in peripheral venous plasma from men and women. The procedure involves: (i) competitive binding of the analyte and tritiated antigen to specific antibodies; (ii) enzymatic conjugation of the free ligand with glucuronic acid; and (iii) separation of the antibody-bound and free ligand by partition of the reactants into an organic and aqueous phase. The technique has been called ligand differentiation immunoassay (LIDIA). The mean sensitivity was 5 pg/tube (equivalent to 0.35 nmol/l female plasma; 0.87 nmol/l male plasma). The mean reagent blank (+/- SD) was 0.24 (0.12) nmol/l female plasma; 0.61 (0.30) nmol/l male plasma. A precision profile gave values less than 50%; the within batch variation was less than 8.3% and the between batch variation over three months was 12.3%. An accuracy profile between the second and penultimate points on the calibration curve gave values between 77 and 103%. The correlation coefficient 'r' between LIDIA and a heterogeneous radioimmunoassay was 0.92 when applied to plasma from men and 0.95 to samples from women.
URI
http://www.ncbi.nlm.nih.gov/pubmed/6855236http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/30517
Citation
A novel pseudohomogeneous radioimmunoassay for the measurement of plasma testosterone. Makawiti DW, Barnard GJ, Matson CM, Collins WP. J Steroid Biochem. 1983 May;18(5):619-23Publisher
Biochemistry, University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10377]