dc.contributor.author | Kiaira, J K | |
dc.contributor.author | Njogu, R M | |
dc.date.accessioned | 2013-06-10T11:48:23Z | |
dc.date.available | 2013-06-10T11:48:23Z | |
dc.date.issued | 1988 | |
dc.identifier.citation | Int J Biochem. 1988;20(10):1165-70. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/3248672 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/30733 | |
dc.description.abstract | 1. The production of pyruvate, glycerol and glycerol-3-phosphate by intact and digitonin-permeabilized Trypanosoma brucei brucei has been studied with glucose or the glycolytic intermediates as substrates. 2. Under aerobic conditions hexosephosphates gave maximal glycolysis in the presence of 40-60 micrograms digitonin/10(8) trypanosomes while the triosephosphates gave it at 20-30 micrograms digitonin/10(8) trypanosomes. 3. In the presence of salicylhydroxamic acid, and the glycolytic intermediates, permeabilized trypanosomes produced equimolar amounts of pyruvate and glycerol-3-phosphate and no glycerol. Under the same conditions, glucose catabolism produced glycerol in addition to pyruvated and glycerol-3-phosphate. 4. In the presence of salicylhydroxamic acid and ATP or ADP intact trypanosomes produced equimolar amounts of pyruvate and (glycerol plus glycerol-3-phosphate) with glucose as substrate. 5. A carrier for ATP and ADP at the glycosomal membrane is implicated. 6. It is apparent that glycerol formation is regulated by the ATP/ADP ratio and that it needs intact glycosomal membrane and the presence of glucose. | en |
dc.language.iso | en | en |
dc.title | Trypanosoma brucei brucei: the catabolism of glycolytic intermediates by digitonin-permeabilized bloodstream trypomastigotes and some aspects of regulation of anaerobic glycolysis | en |
dc.type | Article | en |
local.publisher | Department of Biochemistry, University of Nairobi | en |