dc.contributor.author | Njogu, R M | |
dc.contributor.author | Kiaira, J K | |
dc.date.accessioned | 2013-06-10T11:57:32Z | |
dc.date.available | 2013-06-10T11:57:32Z | |
dc.date.issued | 1982 | |
dc.identifier.citation | Comp Biochem Physiol A Comp Physiol. 1982;71(2):265-9. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/6121651 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/30747 | |
dc.description.abstract | 1. The degree of rat erythrocyte lysis and immobilization of Trypanosoma brucei in infected blood by buffered hypotonic solutions of sodium chloride and sources was studied. 2. At 0.3% sodium chloride solution 98% hemolysis of erythrocytes was achieved while 95% of the original bloodstream trypomastigotes survived and were found to be motile and viable for biochemical study. 3. Further increase in the concentration of sodium chloride above 0.3% revealed an increase in the immobilization of trypanosomes and a decrease in the erythrocyte hemolysis. 4. Bloodstream trypomastigotes have been prepared by differential osmotic lysis of infected blood in 0.3% sodium chloride solution and used for studying their metabolism. | en |
dc.language.iso | en | en |
dc.title | Trypanosoma brucei: a quick method for separating blood-stream trypomastigotes from infected blood by differential osmotic lysis | en |
dc.type | Article | en |
local.publisher | Department of Biochemistry, University of Nairobi | en |