Identification of carbohydrates on Eimeria stiedai sporozoites and their role in the invasion of cultured cells in vitro
Date
1999-02Author
Njenga, Munene John
Zea, Manuel Eduardo Rodriguez
Kawano, Tomomi
Omata, Yoshitaka
Saito, Atsushi
Toyoda, Yutaka
Mikami, Takeshi
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The carbohydrates present on Eimeria stiedai sporozoites and their functional role in the process of invasion of host cells were examined. Lectin-binding sites on the surface of sporozoites were detected by means of peroxidase-conjugated lectins. Sporozoites showed specific binding with UEA-I and PNA lectins, which bind L-fucose and D-galactose, respectively. Exposure of sporozoites to 100 μg/ml UEA-I significantly reduced their ability to invade primary rabbit liver biliary epithelial cells, but similar treatment with PNA had no such effect. Pre-incubation of these cells in Dulbecco's minimum essential medium containing 10% fetal bovine serum and 1% L-fucose suppressed the invasion activity of the sporozoites, but pre-incubation of the sporozoites in the same medium without L-fucose had no effect on cell penetration. D-galactose added to the medium had no effect on the invasion activity of sporozoites. These results indicate that L-fucose residues on E. stiedai sporozoites and L-fucose-binding sites on host cells both are associated with the recognition and/or invasion process.
URI
http://www.sciencedirect.com/science/article/pii/S0304401798002398http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31605
Citation
Veterinary Parasitology Volume 81, Issue 2, 25 February 1999, Pages 99–105Publisher
Univesity of Nairobi Department of Agricultural Economics