dc.contributor.author | Njenga, Munene John | |
dc.contributor.author | Zea, Manuel Eduardo Rodriguez | |
dc.contributor.author | Kawano, Tomomi | |
dc.contributor.author | Omata, Yoshitaka | |
dc.contributor.author | Saito, Atsushi | |
dc.contributor.author | Toyoda, Yutaka | |
dc.contributor.author | Mikami, Takeshi | |
dc.date.accessioned | 2013-06-11T12:38:42Z | |
dc.date.available | 2013-06-11T12:38:42Z | |
dc.date.issued | 1999-02 | |
dc.identifier.citation | Veterinary Parasitology Volume 81, Issue 2, 25 February 1999, Pages 99–105 | en |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0304401798002398 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31605 | |
dc.description.abstract | The carbohydrates present on Eimeria stiedai sporozoites and their functional role in the process of invasion of host cells were examined. Lectin-binding sites on the surface of sporozoites were detected by means of peroxidase-conjugated lectins. Sporozoites showed specific binding with UEA-I and PNA lectins, which bind L-fucose and D-galactose, respectively. Exposure of sporozoites to 100 μg/ml UEA-I significantly reduced their ability to invade primary rabbit liver biliary epithelial cells, but similar treatment with PNA had no such effect. Pre-incubation of these cells in Dulbecco's minimum essential medium containing 10% fetal bovine serum and 1% L-fucose suppressed the invasion activity of the sporozoites, but pre-incubation of the sporozoites in the same medium without L-fucose had no effect on cell penetration. D-galactose added to the medium had no effect on the invasion activity of sporozoites. These results indicate that L-fucose residues on E. stiedai sporozoites and L-fucose-binding sites on host cells both are associated with the recognition and/or invasion process. | en |
dc.language.iso | en | en |
dc.publisher | Univesity of Nairobi | en |
dc.title | Identification of carbohydrates on Eimeria stiedai sporozoites and their role in the invasion of cultured cells in vitro | en |
dc.type | Article | en |
local.publisher | Department of Agricultural Economics | en |