dc.contributor.author | Chohan, BH | |
dc.contributor.author | Taylor, H | |
dc.contributor.author | Obrigewitch, R | |
dc.contributor.author | Lavreys, L | |
dc.contributor.author | Richardson, BA | |
dc.contributor.author | Mandaliya, KN | |
dc.contributor.author | Bwayo, JJ | |
dc.contributor.author | Kreiss, JK | |
dc.contributor.author | Morrow, RA | |
dc.date.accessioned | 2013-06-11T12:45:16Z | |
dc.date.available | 2013-06-11T12:45:16Z | |
dc.date.issued | 2004-06 | |
dc.identifier.citation | J Clin Virol. 2004 Jun;30(2):137-44 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/15125869 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31613 | |
dc.description.abstract | Background:Human herpesvirus 8 (HHV-8) antibody tests vary in reported sensitivity and specificity, depending on the population tested and the assay.
Objectives:The purpose of this study was to compare the ability to detect seroconversion to HHV-8 in a cohort of HHV-8 seronegative female commercial sex workers in Kenya using three tests: HHV-8 viral lysate-based enzyme-linked immunosorbent assay (ELISA), an immunofluorescence assay for HHV-8 lytic antigens (IFA-lytic) and IFA for latent nuclear antigens (IFA-LANA). Study design: By ELISA, 16 women from a prospective cohort of commercial sex workers were identified as seroconverting to HHV-8. A total of 124 post-enrollment samples from these 16 women as well as the enrollment samples were tested for HHV-8 antibodies by all three assays to monitor seroconversion. Results:Of 16 women with apparent seroconversion by ELISA, 8 had a rise in IFA-lytic titers either concomitant with or prior to the first positive ELISA sample and no initial LANA by IFA. Five of the 16 women were IFA-LANA positive at entry, indicating prior infection with HHV-8. Three women had no evidence of seroconversion by either IFA-lytic or IFA-LANA and two of these three had increased ELISA reactivity concomitant with HIV-1 infection.
Conclusions:Conversion from a negative to a positive ELISA result for HHV-8 antibody indicated seroconversion in only half of the study cohort of 16 women when IFA-lytic and IFA-LANA results were considered. The IFA-lytic assay was more sensitive than ELISA for early antibody responses. The IFA-LANA was positive in some women who had neither IFA-lytic nor ELISA antibodies suggesting it may be a marker for latent infections. Presumptive identification of incident HHV-8 infection by ELISA screening followed by IFA-lytic testing to confirm the positive test and IFA-LANA to rule out prior infection provides the most accurate documentation of HHV-8 seroconversion | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Human herpesvirus 8 seroconversion in Kenyan women by enzyme-linked immunosorbent assay and immunofluorescence assay | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya | en |