| dc.contributor.author | Gathuru, JK | |
| dc.contributor.author | Miyoshi, I | |
| dc.contributor.author | Naiki, M | |
| dc.date.accessioned | 2013-06-11T15:15:49Z | |
| dc.date.available | 2013-06-11T15:15:49Z | |
| dc.date.issued | 1991-03 | |
| dc.identifier.citation | J Immunol Methods. 1991 Mar 1;137(1):95-102. | en |
| dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/2010622 | |
| dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/31802 | |
| dc.description.abstract | A procedure for in vitro immunization of splenic lymphocytes with a glycolipid antigen is described. Culture medium supernatant of ConA- and PHA-stimulated spleen cells and that of Con A-stimulated human Jurkat T cell line (IL-2-rich medium) were used as sources of cytokines to support T and B cell stimulation, and anti-mu was used to support B cell differentiation. Unprimed rat spleen cells (2 x 10(6)/ml) were stimulated with 2 micrograms/ml Forssman glycolipid antigen coupled to Sepharose for 4 days. The cells were fused with a mouse myeloma cell line P3-X63-Ag8-U1. At initial screening, 12% of the colony forming wells were secreting specific antibody. After cloning, a stable hybridoma cell line (designated 4C3) was established which secreted a monoclonal IgM antibody directed against the carbohydrate moiety of Forssman glycosphingolipid (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc-ceramide). | en |
| dc.language.iso | en | en |
| dc.publisher | University of Nairobi | en |
| dc.title | In vitro immunization of rat spleen lymphocytes with Forssman glycosphingolipid antigen and the generation of a monoclonal antibody | en |
| dc.type | Article | en |
| local.publisher | School of Public Health, University of Nairobi | en |
