dc.contributor.author | Walker, LM | |
dc.contributor.author | Phogat, SK | |
dc.contributor.author | Chan-Hui, PY | |
dc.contributor.author | Wagner, D | |
dc.contributor.author | Phung, P | |
dc.contributor.author | Goss, JL | |
dc.contributor.author | Wrin, T | |
dc.contributor.author | Simek, MD | |
dc.contributor.author | Fling, S | |
dc.contributor.author | Mitcham, JL | |
dc.contributor.author | Lehrman, JK | |
dc.contributor.author | Priddy, FH | |
dc.contributor.author | Olsen, OA | |
dc.contributor.author | Frey, SM | |
dc.contributor.author | Hammond, PW | |
dc.contributor.author | Protocol G Principal Investigators | |
dc.contributor.author | Kaminsky, S | |
dc.contributor.author | Zamb, T | |
dc.contributor.author | Moyle, M | |
dc.contributor.author | Koff, WC | |
dc.contributor.author | Poignard, P | |
dc.contributor.author | Burton, DR | |
dc.date.accessioned | 2013-06-12T13:53:28Z | |
dc.date.available | 2013-06-12T13:53:28Z | |
dc.date.issued | 2009-10 | |
dc.identifier.citation | Science. 2009 Oct 9;326(5950):285-9. doi: 10.1126/science.1178746. Epub 2009 Sep 3 | en |
dc.identifier.uri | mwww.ncbi.nlm.nih.gov/whalecom0/pubmed/19729618 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/32413 | |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335270/ | |
dc.description.abstract | Broadly neutralizing antibodies (bNAbs), which develop over time in some HIV-1-infected individuals, define critical epitopes for HIV vaccine design. Using a systematic approach, we have examined neutralization breadth in the sera of about 1800 HIV-1-infected individuals, primarily infected with non-clade B viruses, and have selected donors for monoclonal antibody (mAb) generation. We then used a high-throughput neutralization screen of antibody-containing culture supernatants from about 30,000 activated memory B cells from a clade A-infected African donor to isolate two potent mAbs that target a broadly neutralizing epitope. This epitope is preferentially expressed on trimeric Envelope protein and spans conserved regions of variable loops of the gp120 subunit. The results provide a framework for the design of new vaccine candidates for the elicitation of bNAb responses. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target | en |
dc.type | Article | en |
local.publisher | School of medicine,University of Nairobi | en |
local.publisher | Department of Immunology and Microbial Science | en |