HIV-1-specific cellular immune responses among HIV-1-resistant sex workers
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Date
2000Author
Fowke, R K
Kaul, R
Type
ArticleLanguage
enMetadata
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The goal of the present study was to determine whether there were HIV-1 specific cellular immune responses among a subgroup of women within a cohort of Nairobi prostitutes (n = 1800) who, despite their intense sexual exposure to HIV-1, are epidemiologically resistant to HIV-1 infection. Of the 80 women defined to be resistant, 24 were recruited for immunological evaluation. The HIV-1-specific T-helper responses were determined by IL-2 production following stimulation with HIV-1 envelope peptides and soluble gp120. Cytotoxic T lymphocyte responses were determined by lysis of autologous EBV-transformed B cell lines infected with control vaccinia virus or recombinant vaccinia viruses containing the HIV-1 structural genes env, gag and pol. Resistant women had significantly increased HIV-1 specific T-helper responses, as determined by in vitro IL-2 production to HIV-1 envelope peptides and soluble glycoprotein 120, compared with low-risk seronegative and HIV-1-infected controls (P < or = 0.01, Student's t-test). Seven of the 17 (41%) resistant women showed IL-2 stimulation indices > or = 2.0. HIV-1-specific CTL responses were detected among 15/22 (68.2%) resistant women compared with 0/12 low-risk controls (Chi-squared test, P < 0.001). In the two resistant individuals tested, the CTL activity was mediated by CD8+ effectors. Many HIV-1-resistant women show evidence of HIV-1-specific T-helper and cytotoxic responses. These data support the suggestion that HIV-1-specific T-cell responses contribute to protection against HIV-1 infection.
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/32552http://www.ncbi.nlm.nih.gov/pubmed/11114968
Citation
12. Fowke KR, Kaul R, RKLOKRWJNNJBTBBJJSJNSGMJJ. 2000. HIV-1-specific cellular immune responses among HIV-1-resistant sex workers. Immunol Cell Biol.Publisher
University of Nairobi School of Public Health Centre for HIV Prevention and Research
Collections
- Faculty of Health Sciences (FHS) [10378]