Biocatalysis at 90 o C in Microemulsions using Crosslinked Enzyme-Polylysine Films
Date
2007Author
Rusling, James F
Kumar, Challa V
Guto, Peterson M
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Biocatalysis is expected to play
a significant role in future
industrial processes, especially in regio- and
stereoselective syntheses.
1
Concerted effort has been
directed toward discovering en
zymes that operate at high
temperatures to improve cat
alytic efficiency, including
isolation from thermophilic bacteria, chemical
modification, directed evolution,
2
and immobilization on
specialized solid supports.
3
Nevertheless, thermophilic
biocatalysts are rarely usable
at temperatures above 60 or
70
o
C. This paper discusses redox enzymes crosslinked
into a poly-L-lysine (PLL) matrix attached to
carboxylated surfaces that provides th
ermostable films
designed for biocatalysis in microemuslions at 90
o
C.
Citation
211th ECS Meeting 2007Publisher
The Electrochemical Society Department of Chemistry, University of Connecticut, Storrs CT 06269, USA Department of Pharmacology, University of Connecticut Health Cent er, Farmington, CT 06032