Biocatalysis at 90 o C in Microemulsions using Crosslinked Enzyme-Polylysine Films

Abstract

Biocatalysis is expected to play a significant role in future industrial processes, especially in regio- and stereoselective syntheses. 1 Concerted effort has been directed toward discovering en zymes that operate at high temperatures to improve cat alytic efficiency, including isolation from thermophilic bacteria, chemical modification, directed evolution, 2 and immobilization on specialized solid supports. 3 Nevertheless, thermophilic biocatalysts are rarely usable at temperatures above 60 or 70 o C. This paper discusses redox enzymes crosslinked into a poly-L-lysine (PLL) matrix attached to carboxylated surfaces that provides th ermostable films designed for biocatalysis in microemuslions at 90 o C.