| dc.description.abstract | The ELISA and an immunoblotting technique were used to study F38-type
mycoplasmas - an important cause of contagious caprine pleuropneumonia - and
a number of related mycoplasma species, subspecies, types or serogroups.
Two-way ELISA cross-reactivity was demonstrated between five mycoplasmas,
namely strain F38, Mycoplasma mycoides subsp. mycoides (LC strain), M. equigenitalium, M. primatum and bovine serogroup 7. In addition one-way crossreactivity was demonstrated between F38 and each of the following mycoplasmas:
M. mycoides subsp. mycoides (two SC strains), M. mycoides subsp. capri, and
bovine serogroup L. F38 and M. capricolum did not cross-react.
Immunoblot analysis, unlike ELISA, revealed that F38 and M. capricolum were
closely related. At least four major protein antigens were shared between F38,
M. mycoides subsp. mycoides (SC and LC strains), M. mycoides subsp. capri and
bovine serogroup 7. The ELISA cross-reactions (above) shown by M. equigenitalium and M. primatum with each other, with F38 and with other mycoplasmas
were not apparent by immunoblotting. | en |
