Isolation of high quality DNA and RNA from cambium of the East African greenheart (Warburgia ugandensis)
Date
2009Author
Muge, E
Burg, K
Kadu, C
Muchugi, A
Lemurt, S
Jamnadass, R
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The genus Warburgia belongs to the Canellaceae, a small family of tropical aromatic tree species important for their antibacterial and antifungal medicinal qualities amongst many other functions. Many published protocols for nucleic acid isolation failed to yield sufficiently good quality amounts for analysis. We have therefore developed a simple and fast CTAB (cetyl-trimethyl ammonium bromide)-based extraction protocol for routine isolation of high-quality nucleic acids. Good quality genomic DNA
(gDNA) and RNA was obtained and was suitable for PCR amplification, restriction endonuclease digestion, Southern blotting and RT-PCR, respectively.
URI
http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/36865http://www.ajol.info/index.php/ajb/article/view/60982
Citation
KIRWA, DR MUGE EDWARD. 2009. Muge, E., Burg, K., Kadu, C., Muchugi, A., Lemurt, S and Jamnadass, R 2009. Isolation of High Quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis). African Journal of Biotechnology Vol. 8 (13), pp. 3036-3040.. The Academic Journal of Daystar university.. : African Journal of Biotechnology Vol. 8 (13), pp. 3036-3040Publisher
University of Nairobi, College of Health Science
Collections
- Faculty of Health Sciences (FHS) [10377]