Protective Effect Of Whey Protein Hydrolysates Against Hydrogen Peroxide-induced Oxidative Stress On Pc12 Cells
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Date
2012-11Author
Zhang, Qiu-Xiang
Ling, Yu-Fang
Sun, Zhen
Zhang, Li
Yu, Hui-Xin
Kamau, Samuel Mburu
Lu, Rong-Rong
Type
ArticleLanguage
enMetadata
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The protective effect of whey protein hydrolysates (WPHs) against H2O2-induced oxidative damage on rat pheochromocytoma line 12 (PC12) cells was studied. Whey protein was hydrolyzed by pepsin and trypsin and purified by macrospore absorption resins. PC12 cells were pretreated with WPHs (from 369 to 1,980 Da) at different concentrations for 2 h, then washed and incubated with 100 μM H2O2 in the presence of WPHs for another 24 h. With 100–400 μg WPH/ml the viable cells increased by 20–30 % when incubated with H2O2 suggesting that they may play a role as antioxidant in foods.
URI
http://link.springer.com/article/10.1007/s10529-012-1017-1http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/38750
Citation
Biotechnology Letters November 2012, Volume 34, Issue 11, pp 2001-2006Publisher
University of Nairobi College of Humanities and Social Sciences