Modified Granada Agar Medium For The Detection Of Group B Streptococcus Carriage In Pregnant Women.
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Date
2001Author
Claeys, G
Verschraegen, G
Temmerman, M
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
OBJECTIVES:
To improve the detection rate of group B streptococci (GBS) in pregnant women, aiming at the prevention of early-onset septicemia in the newborn.
METHODS:
The yield from culturing two sites, vaginal and anorectal, on a Modified Granada Medium (MGM) was compared with our standard approach of culturing a vaginal swab on blood agar (BA).
RESULTS:
Samples were processed from 430 consecutive pregnant women. GBS was isolated from the vagina in 11.6% with BA, and in 13.7% with MGM. In 17.0% of anorectal samples, GBS was identified with MGM. The combination of both sites and media had a yield of 20.0%. MGM identified all but six (2%) of 310 GBS strains after aerobic incubation, with use of a cover slide, and missed only three strains (1%) after anaerobic incubation.
CONCLUSIONS:
Separate culture of vaginal and anorectal samples using the same MGM agar plate resulted in an increase in detection rate for GBS of 76% as compared to BA alone. The technique is simple and results are available after overnight incubation. MGM was confirmed as a specific medium for the identification of GBS, with a sensitivity of 98-99%
URI
http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/11284939http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/40661
Citation
Clin Microbiol Infect. 2001 Jan;7(1):22-4Publisher
University of Nairobi Community Health ,School of Health Sciences
Collections
- Faculty of Health Sciences (FHS) [10377]