dc.contributor.author | Ji, H | |
dc.contributor.author | Ball, TB | |
dc.contributor.author | Liang, BB | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Plummer, FA. | |
dc.date.accessioned | 2013-06-28T14:09:32Z | |
dc.date.available | 2013-06-28T14:09:32Z | |
dc.date.issued | 2008-06 | |
dc.identifier.citation | DNA Seq. 2008 Jun;19(3):326-31 | en |
dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/17852336 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/41898 | |
dc.description.abstract | Interferon regulatory factor-1 (IRF-1) plays important roles in host immunity, cell proliferation and apoptosis. The current GenBank sequence for human IRF-1 (accession number: L05072) was derived from a human placenta DNA library and reported in 1992. In one recent population-based sequence study, we observed consistent discrepancies between our IRF-1 sequence data and GenBank reference sequences suggesting that, current IRF-1 reference sequence was not representative for all populations. By complete gene sequencing, we obtained a representative full-length IRF-1 sequence from a single subject. Compared to submission L05072, our population-based data contains: 35 nucleotide additions, 8 nucleotide removals and another 12 nucleotide replacements. A single nucleotide difference was observed in the IRF-1 promoter sequence compared to GenBank sequence (X53095). These changes were confirmed in 350 Kenyans and 28 non-African donors. The accuracy of a reference sequence is crucial for downstream genetic and functional studies and this study provides more complete and accurate data on the sequence of the human IRF-1 gene and its immediate promoter region. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Human Interferon Regulatory Factor-1 Gene And Its Promoter Sequences Revealed By Population-based Complete Gene Sequencing. | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology | en |