Cultivation of Plasmodium falciparum parasites in a serum-free medium
Date
1993-09Author
Ofulla, AV
Okoye, VC
Khan, B
Githure, JI
Roberts, CR
Johnson, AJ
Martin, SK
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
The elimination of serum from Plasmodium falciparum culture media could decrease costs, enhance procurement, and improve the feasibility of large-scale production of parasite material. We provide a semi-defined, serum-free formulation, of commercially available constituents that supports P. falciparum parasite growth at rates comparable with those obtained with serum-supplemented media. The medium is composed of RPMI 1640 to which HEPES, extra glucose, bicarbonate, and hypoxanthine have been added. Bovine albumin and serum-derived, lipids-cholesterol-rich mixture are then used in place of serum.
URI
http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/8396859http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/42349
Citation
Am J Trop Med Hyg. 1993 Sep;49(3):335-40.Publisher
University of Nairobi. Department of Zoology