Cultivation of Plasmodium falciparum parasites in a serum-free medium
dc.contributor.author | Ofulla, AV | |
dc.contributor.author | Okoye, VC | |
dc.contributor.author | Khan, B | |
dc.contributor.author | Githure, JI | |
dc.contributor.author | Roberts, CR | |
dc.contributor.author | Johnson, AJ | |
dc.contributor.author | Martin, SK | |
dc.date.accessioned | 2013-06-29T10:31:32Z | |
dc.date.available | 2013-06-29T10:31:32Z | |
dc.date.issued | 1993-09 | |
dc.identifier.citation | Am J Trop Med Hyg. 1993 Sep;49(3):335-40. | en |
dc.identifier.uri | http://hinari-gw.who.int/whalecomwww.ncbi.nlm.nih.gov/whalecom0/pubmed/8396859 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/42349 | |
dc.description.abstract | The elimination of serum from Plasmodium falciparum culture media could decrease costs, enhance procurement, and improve the feasibility of large-scale production of parasite material. We provide a semi-defined, serum-free formulation, of commercially available constituents that supports P. falciparum parasite growth at rates comparable with those obtained with serum-supplemented media. The medium is composed of RPMI 1640 to which HEPES, extra glucose, bicarbonate, and hypoxanthine have been added. Bovine albumin and serum-derived, lipids-cholesterol-rich mixture are then used in place of serum. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi. | en |
dc.title | Cultivation of Plasmodium falciparum parasites in a serum-free medium | en |
dc.type | Article | en |
local.publisher | Department of Zoology | en |
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