dc.contributor.author | Kato, Shiro | |
dc.contributor.author | Okabe, Masahiro | |
dc.contributor.author | Nishiura, Katsumi | |
dc.contributor.author | Hashimoto, Masato | |
dc.contributor.author | Naiki, Masaharu | |
dc.contributor.author | Mukuria, Joseph C | |
dc.date.accessioned | 2013-06-29T14:22:16Z | |
dc.date.available | 2013-06-29T14:22:16Z | |
dc.date.issued | 1985-06 | |
dc.identifier.citation | Journal of Immunological Methods Volume 80, Issue 1, 12 June 1985, Pages 97–106 | en |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/42679 | |
dc.description.abstract | A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein found to have a strong H-D antigen potency. The antigen-antibody complex was precipitated with normal human serum as the carrier protein, followed by the addition of rabbit anti-human IgG F(ab')2 serum. With this method, different H-D antigen-active molecules were compared for heterophile H-D antigen potency with reasonable sensitivity detecting about 0.3 ng of cold glycoprotein. 8 different lung cancer tissues were assayed for H-D antigen. The sera from the 8 lung cancer patients were also screened by ELISA and RIA in an attempt to correlate expression of H-D antigen on tissues with elevation of H-D antibodies. The results showed that all patients' tissues expressed the antigen(s) but only 3 of them had abnormal levels of H-D antibodies. This could have been due to excess antigens in circulation or immune complexes | en |
dc.language.iso | en | en |
dc.subject | RIA | en |
dc.subject | inhibition | en |
dc.subject | heterophile | en |
dc.subject | Hanganutziu-Deicher | en |
dc.subject | ELISA | en |
dc.subject | carrier protein | en |
dc.title | A potential radioimmunoassay system for detection of Hanganutziu-Deicher type heterophile antigen (s) and antibodies in tissues and fluids | en |
dc.type | Article | en |
local.publisher | Department of Biochemistry | en |