Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae
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Date
1987Author
Coon, HG.
Kantoff, P,
Gwadz, RW,
Romans, P,
Sakai, RK,
Miller, LH,
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
Foreign DNA was successfully introduced into the germline of the African mosquito vector of malaria Anopheles gambiae. Stable integration of genes into the germlines of insects had been achieved previously only in Drosophila melanogaster and related species and required the use of the P element transposon. In these experiments with Anopheles gambiae, the plasmid pUChsneo was used, which contains the selectable marker neo gene flanked by P element inverted repeats. Mosquitoes injected with this plasmid were screened for resistance to the neomycin analog G-418. A single event of plasmid insertion was recovered. Integration appears to be stable and, thus far, resistance to G-418 has been expressed for eight generations. The transformation event appears to be independent of P.
Citation
Miller, LH, Sakai RK, Romans P, Gwadz RW, Kantoff P, Coon HG. 1987. Stable integration and expression of a bacterial gene in the mosquito Anopheles gambiae., 1987 Aug 14. Science (New York, N.Y.). 237(4816):779-81.Publisher
Department of Paediatrics & Child Health, University o0f Nairobi
Collections
- Faculty of Health Sciences (FHS) [10377]