dc.contributor.author | Ji, Hezhao | |
dc.contributor.author | Bal, Terry Blake | |
dc.contributor.author | Ao, Zhujun | |
dc.contributor.author | Kimani, Joshua | |
dc.contributor.author | Yao, Xiaojian | |
dc.contributor.author | Plummer, FA | |
dc.date.accessioned | 2013-07-27T09:51:44Z | |
dc.date.available | 2013-07-27T09:51:44Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Scandinavian Journal of Infectious Diseases May 2010, Vol. 42, No. 5 , Pages 389-394 | en |
dc.identifier.uri | http://informahealthcare.com/doi/abs/10.3109/00365540903496536 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/51727 | |
dc.description.abstract | We previously described the polymorphism in the interferon regulatory factor-1 (IRF-1) gene as a novel correlate of resistance to HIV-1 infection in a Kenyan female sex worker cohort. However, the underlying mechanisms likely mediating this association remained to be elucidated. The initiation of HIV-1 long terminal repeat (LTR) transcription in peripheral blood mononuclear cells (PBMCs) from subjects with different IRF-1 haplotypes, representing protective, intermediate and the least protective IRF-1 allele combinations, were investigated here. A single-cycle pseudovirus construct expressing vesicular stomatitis virus envelop G-protein (VSV-G) and having an HIV-1 pNL4.3 backbone with luciferase insert was used to infect PBMCs with different IRF-1 haplotypes. The efficiency of early HIV-1 LTR transcription was monitored using a luciferase assay. IRF-1 protein levels induced by the infection were measured by quantitative Western blot. Our results showed that PBMCs with the protective IRF-1 genotype demonstrated significantly lower HIV-1 LTR transcription during the initial stages of infection compared to PBMCs with other haplotypes, which correlated with the kinetics of IRF-1 responsiveness to HIV-1 infection in the cells. It suggests that IRF-1 genotypes alter the efficiency of early HIV-1 LTR transcription, likely via modulating expression of IRF-1. This may represent one mechanism mediating the association between IRF-1 polymorphisms and resistance to HIV-1 infection | en |
dc.language.iso | en | en |
dc.title | Reduced HIV-1 long terminal repeat transcription in subjects with protective interferon regulatory factor-1 genotype: A potential mechanism mediating resistance to infection by HIV-1 | en |
dc.type | Article | en |
local.publisher | Department of Microbiology, University of Nairobi, Nairobi, Kenya | en |
local.publisher | National Microbiology Laboratories, Public Health Agency of Canada, Winnipeg, Manitoba, Canada | en |
local.publisher | Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada | en |