Genetic analysis of human DNA recovered from minute amounts of serum or plasma
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Date
1995-03-13Author
Fowke, Keith R
Plummer, Francis A
Simonsen, J Neil
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
A rapid and inexpensive method is described where a small amount of serum or plasma was used as the source of DNA for genetic analysis. Using a silica gel matrix DNA was isolated from 50 μl of archived serum or plasma. The specimens were collected from 13 individuals at two separate time points 3–6 years apart. The polymorphic region of second exon of the MHC class II gene HLA DQA1 was amplified using the polymerase chain reaction (PCR) to sufficient quantities to permit genetic analysis using allele-specific oligonucleotides (ASO). Allelic typing of each specimen was performed and the reproducibility of the method was demonstrated in that in all 13 cases the two independently isolated specimens produced the identical ASO binding patterns. No qualitative difference was noted in the amplified product generated from plasma or serum. This study demonstrates (a) that minute amounts of serum or plasma are able to provide sufficient quantity and quality of DNA to permit genetic analyses (b) and that the source of serum can be archived for many years.
URI
http://www.sciencedirect.com/science/article/pii/002217599400297Ahttp://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/53984
Citation
Journal of Immunological Methods Volume 180, Issue 1, 13 March 1995, Pages 45–51Publisher
University of Nairobi Department of Medical Microbiology
Collections
- Faculty of Health Sciences (FHS) [10377]