Liquid chromatographic separation of hexopyranosylated cytosine nucleosides from their degradation products
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Date
1997-11Author
Thoithi, G
Van, Schepdael A
Herdewijn, P
Roets, E
Hoogmartens, J
Type
ArticleLanguage
enMetadata
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Development of a liquid chromatographic method which can separate each of a series of hexopyranosylated cytosine nucleosides from their degradation products formed at acid, neutral and basic pH is described. Both silica-based reverse-phase and polymer columns were examined. Influence of the mobile phase pH, ion-pairing agent, concentration of the buffer and type and concentration of organic modifier were systematically investigated. The concentration of the ion-pairing agent and the buffer were found to have a major effect on selectivity. Samples were finally analyzed on a poly(styrene-divinylbenzene), PLRP-S 100 A (8 microns) 250 x 4.6 mm I.D. column at 60 degrees C and with a mobile phase consisting of acetonitrile-sodium octanesulphonate (pH 2.5; 0.02 M)-potassium phosphate buffer (pH 2.5; 0.2 M)-water (X:25:50:25-X, v/v, where X is variable).
URI
http://www.ncbi.nlm.nih.gov/pubmed/9589414http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/57773
Citation
J Pharm Biomed Anal. 1997 Nov;16(3):533-40Publisher
University of Nairobi school of public health
Collections
- Faculty of Health Sciences (FHS) [10387]