dc.contributor.author | Söderlund, J | |
dc.contributor.author | Hirbod, T | |
dc.contributor.author | Smed-Sörensen, A | |
dc.contributor.author | Johansson, U | |
dc.contributor.author | Kimani, J | |
dc.contributor.author | Plummer, F | |
dc.contributor.author | Spetz, AL | |
dc.contributor.author | Andersson, J | |
dc.contributor.author | Kaul, R | |
dc.contributor.author | Broliden, K. | |
dc.date.accessioned | 2013-11-26T11:21:14Z | |
dc.date.available | 2013-11-26T11:21:14Z | |
dc.date.issued | 2007-01-23 | |
dc.identifier.citation | AIDS Res Hum Retroviruses. 2007 Jan;23(1):101-6. | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/17263639 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/60445 | |
dc.description.abstract | Highly exposed persistently seronegative (HEPS) individuals have previously been shown to mount HIV-1-specific humoral and cellular immune responses in the mucosa, despite their uninfected status. It is thus possible that HEPS individuals are protected from HIV-1 infection at the mucosal level. Recent work supports the hypothesis that dendritic cells are involved in the establishment of a mucosal HIV-1 infection as well as the dissemination to other target cells. However, no previous study has investigated if samples collected from HEPS individuals have the capacity to prevent HIV-1 infection in the presence of dendritic cells in vitro. We therefore established an assay that measures HIV-1 neutralization in cocultures of HIV-1-exposed dendritic cells (DC) and PBMC. Plasma and cervicovaginal lavage (CVL) samples from HIV-1-infected patients and HEPS individuals, enrolled in a well-characterized sex worker cohort in Kenya, were evaluated. Most plasma and CVL samples of HIV-1-infected patients neutralized HIV-1 in the DC/PBMC cocultures. Neither plasma nor CVL samples of most HEPS individuals had this capacity. However, they readily neutralized HIV-1 infection of PBMC alone. This may suggest that protection against HIV-1 infection in HEPS individuals occurs prior to interaction between HIV-1-exposed DC and other target cells. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Plasma and mucosal fluid from HIV type 1-infected patients but not from HIV type 1-exposed uninfected subjects prevent HIV type 1-exposed DC from infecting other target cells. | en |
dc.type | Article | en |
local.publisher | Department of Medical Microbiology | en |