dc.contributor.author | Nykoluk, M | |
dc.contributor.author | Bailey, RC | |
dc.contributor.author | Moses, S | |
dc.contributor.author | Plummer, FA | |
dc.contributor.author | Luo, M. | |
dc.date.accessioned | 2013-11-26T14:11:20Z | |
dc.date.available | 2013-11-26T14:11:20Z | |
dc.date.issued | 2013-08 | |
dc.identifier.citation | Tissue Antigens. 2013 Aug;82(2):131-3. doi: 10.1111/tan.12157 | en |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/23849069 | |
dc.identifier.uri | http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/60534 | |
dc.description.abstract | Three novel human leukocyte antigen (HLA) alleles were identified using a sequence-based typing of HLA class I and class II alleles of 1867 participants from a male circumcision cohort in Kenya. The new alleles were first identified by sequencing and then confirmed by cloning the polymerase chain reaction (PCR) products and sequencing multiple clones. HLA-B*58:43 was identical to HLA-B*58:02 with the exception of a nucleotide change at codon 125 in exon 3 (GCC→ACC), and resulted in the amino acid change from Alanine to Threonine. HLA-C*03:190 was identical to HLA-C*03:02:01 with the exception of a nucleotide change at codon 131 in exon 3 (CGC→TGC), and resulted in the amino acid change from Arginine to Cysteine. HLA-DPA1*01:12 was identical to HLA-DPA1*01:03:01:01 with the exception of a nucleotide change at codon 66 in exon 2 (TTG→TCG), and resulted in the amino acid change from Leucine to Serine. | en |
dc.language.iso | en | en |
dc.publisher | University of Nairobi | en |
dc.title | Identification of three novel human leukocyte antigen alleles, HLA-B58:43 HLA-C03:190 and HLA-DPA101:12 in an East African cohort. | en |
dc.type | Article | en |
local.publisher | HIV and Human Genetics, National Microbiology Laboratory | en |