Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.

dc.contributor.author	Kanyara, JN
dc.contributor.author	Njagi, EN
dc.date.accessioned	2013-12-04T12:47:52Z
dc.date.available	2013-12-04T12:47:52Z
dc.date.issued	2005
dc.identifier.citation	Phytother Res. 2005 Apr;19(4):287-90.	en_US
dc.identifier.uri	http://www.ncbi.nlm.nih.gov/pubmed/16041768
dc.identifier.uri	http://hdl.handle.net/11295/61757
dc.description.abstract	An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented	en_US
dc.language.iso	en	en_US
dc.publisher	Wiley	en_US
dc.title	Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.	en_US
dc.type	Article	en_US