Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.
dc.contributor.author | Kanyara, JN | |
dc.contributor.author | Njagi, EN | |
dc.date.accessioned | 2013-12-04T12:47:52Z | |
dc.date.available | 2013-12-04T12:47:52Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Phytother Res. 2005 Apr;19(4):287-90. | en_US |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/pubmed/16041768 | |
dc.identifier.uri | http://hdl.handle.net/11295/61757 | |
dc.description.abstract | An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented | en_US |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.title | Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay. | en_US |
dc.type | Article | en_US |
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