Natural killer cell and T-cell subset distributions and activation influence susceptibility to perinatal HIV-1 infection
Date
2014Author
Wamalwa, Dalton
Maleche-Obimbo, Elizabeth
Bosire, Rose
Lejarcegui, Nicholas
Itaya, Grace
Kunwar, Pratima
Gasper, Melanie A
Type
ArticleLanguage
enMetadata
Show full item recordAbstract
OBJECTIVE:
To determine neonatal immunologic factors that correlate with mother-to-child-transmission of HIV-1.
DESIGN:
This case-control study compared cord blood natural killer (NK) and T-cell populations of HIV-1 exposed infants who subsequently acquired infection by 1 month (cases) to those who remained uninfected by 1 year of life (controls). Control specimens were selected by proportional match on maternal viral load.
METHODS:
Cryopreserved cord blood mononuclear cells (CBMCs) were thawed and stained for multiparameter flow cytometry to detect NK and T-cell subsets and activation status. CBMCs were also used in a viral suppression assay to evaluate NK cell inhibition of HIV-1 replication in autologous CD4 T cells.
RESULTS:
Cord blood from cases contained a skewed NK cell repertoire characterized by an increased proportion of CD16CD56 NK cells. In addition, cases displayed less-activated CD16CD56 NK cells and CD8 T cells, based on HLA-DRCD38 costaining. NK cell suppression of HIV-1 replication ex vivo correlated with the proportion of acutely activated CD68CD16CD56 NK cells. Finally, we detected a higher proportion of CD27CD45RA effector memory CD4 and CD8 T cells in cord blood from cases compared with controls.
CONCLUSION:
When controlled for maternal viral load, cord blood from infants who acquired HIV-1 had a higher proportion of CD16CD56 NK cells, lower NK cell activation and higher levels of mature T cells (potential HIV-1 targets) than control infants who remained uninfected. Our data provide evidence that infant HIV-1 acquisition may be influenced by both innate and adaptive immune cell phenotypes and activation status.