Identification Of A Synthetic Peptide Inducing Cross-reactive Antibodies Binding To Rhipicephalus (boophilus) Decoloratus, Rhipicephalus (boophilus) Microplus, Hyalomma Anatolicum Anatolicum And Rhipicephalus Appendiculatus Bm86 Homologues
Date
2009Author
Kopp, Nadja
Diaz, Diana
Amacker, Mario
Odongo, David O
Beier, Konstantin
Nitsch, Cordula
Bishop, Richard P
Daubenberger, Claudia
Language
enMetadata
Show full item recordAbstract
The BM86 antigen, originally identified in Rhipicephalus (Boophilus) microplus, is the basis of the only commercialized anti-tick vaccine. The long-term goal of our study is to improve BM86 based vaccines by induction of high levels of tick gut binding antibodies that are also cross-reactive with a range of BM86 homologues expressed in other important tick species. Here we have used a BD86 derived synthetic peptide, BD86-3, to raise a series of mouse monoclonal antibodies. One of these mAbs, named 12.1, recognized BM86 homologues in immuno-histochemical analyses in four out of five tick species including R. (B.) microplus, Rhipicephalus (Boophilus) decoloratus, Hyalomma anatolicum anatolicum and Rhipicephalus appendiculatus. Our results indicate that broadly cross-reactive tick gut binding antibodies can be induced after immunization with a synthetic peptide derived from the protein BD86.
URI
http://www.sciencedirect.com/science/article/pii/S0264410X09014327http://hdl.handle.net/11295/73265
Citation
Vaccine Volume 28, Issue 1, 10 December 2009, Pages 261–269Publisher
University of Nairobi