dc.contributor.author | Kiboi, D M | |
dc.contributor.author | Irungu, B N | |
dc.contributor.author | Langat, B | |
dc.contributor.author | Wittlin, S | |
dc.contributor.author | Brun, R | |
dc.contributor.author | Chollet, J | |
dc.contributor.author | Abiodun, O | |
dc.contributor.author | Nganga, J K | |
dc.contributor.author | Nyambati, V C S | |
dc.contributor.author | Rukunga, G M | |
dc.contributor.author | Bell, A | |
dc.contributor.author | Nzila, A | |
dc.date.accessioned | 2014-07-30T07:12:28Z | |
dc.date.available | 2014-07-30T07:12:28Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Kiboi, D. M., Irungu, B. N., Langat, B., Wittlin, S., Brun, R., Chollet, J., ... & Nzila, A. (2009). < i> Plasmodium berghei</i> ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model. Experimental parasitology, 122(3), 196-202. | en_US |
dc.identifier.uri | http://hdl.handle.net/11295/73354 | |
dc.description.abstract | We have selected piperaquine (PQ) and lumefantrine (LM) resistant Plasmodium berghei ANKA parasite lines in mice by drug pressure. Effective doses that reduce parasitaemia by 90% (ED90) of PQ and LM against the parent line were 3.52 and 3.93 mg/kg, respectively. After drug pressure (more than 27 passages), the selected parasite lines had PQ and LM resistance indexes (I90) [ED90 of resistant line/ED90 of parent line] of 68.86 and 63.55, respectively. After growing them in the absence of drug for 10 passages and cryo-preserving them at −80 °C for at least 2 months, the resistance phenotypes remained stable. Cross-resistance studies showed that the PQ-resistant line was highly resistant to LM, while the LM-resistant line remained sensitive to PQ. Thus, if the mechanism of resistance is similar in P. berghei and Plasmodium falciparum, the use of LM (as part of Coartem®) should not select for PQ resistance. | en_US |
dc.language.iso | en | en_US |
dc.publisher | University of Nairobi | en_US |
dc.subject | Malaria; Plasmodium berghei; Drug resistance; Cross-resistance; Lumefantrine; Piperaquine | en_US |
dc.title | Plasmodium berghei ANKA: Selection of resistance to piperaquine and lumefantrine in a mouse model | en_US |
dc.type | Article | en_US |
dc.type.material | en_US | en_US |