Browsing Journal Articles by Author "Yamagishi, Junya"
Now showing items 1-7 of 7
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Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis
Goo, Youn-Kyoung; Jia, Honglin; Aboge, G. Oluga; Terkawi, M. Alaa; Lee, Eung-goo; Yamagishi, Junya; Nishikawa, Yoshifumi; Jang, Hyung-Kwan; Sunaga, Fujiko; Namikawa, Kazuhiko; Fujisaki, Kozo; Xuan, Xuenan (2009)A novel gene, BgP12, encoding a 12-kDa protein was identified from Babesia gibsoni. The full-length cDNA of BgP12 contains an open reading frame of 378 bp, corresponding to 126 amino acid (aa) residues consisting of a ... -
Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family
Goo, Youn-Kyoung; Aboge, Gabriel Oluga; Terkawi, M. Alaa; Jia, Honglin; Yamagishi, Junya; Sunaga, Fujiko; Namikawa, Kazuhiko; Cha, Se-Yeoun; Jang, Hyung-Kwan; Kim, Suk; Nishikawa, Yoshifumi; Xuan, Xuenan (2012)We determined the molecular characteristics of four proteins, BgP32, BgP45, BgP47, and BgP50, of Babesia gibsoni. Localization by subcellular fractionations followed by Western blotting revealed that the corresponding ... -
Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen
Terkawi, M. A.; Aboge, G. Oluga; Jia, H; Goo, Y K; Ooka, H; Yamagishi, Junya; Nishikawa, Yoshifumi; Shin-ichiro, Kawazu; Fujisaki, K; Xuan, Xuenan (Department of Public Health, Pharmacology & Toxicology, University of Nairobi, 2008)A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 ... -
Macrophages Are Critical for Cross-Protective Immunity Conferred by Babesia microti against Babesia rodhaini Infection in Mice
Li, Yan; Terkawi, Mohamad Alaa; Nishikawa, Yoshifumi; Aboge, Gabriel Oluga; Luo, Yuzi; Ooka, Hideo; Goo, Youn-Kyoung; Yu, Longzheng; Cao, Shinuo; Sun, Yongfeng; Yamagishi, Junya; Masatani, Tatsunori; Yokoyama, Naoaki; Igarashi, Ikuo; Xuan, Xuenan (Department of Public Health, Pharmacology and Toxicology, 2012)AlthoughprimaryinfectionofmicewithBabesiamicrotihasbeenshowntoprotectmiceagainstsubsequentlethalinfection by Babesiarodhaini,themechanismbehindthecross-protection is unknown.To unravel lthis mechanism,we investigated the ... -
Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
Cao, Shinuo; Aboge, Gabriel Oluga; Terkawi, Mohamad Alaa; Yu, Longzheng; Kamyingkird, Ketsarin; Luo, Yuzi; Li, Yan; Goo, Youn-Kyoung; Yamagishi, Junya; Nishikawa, Yoshifumi; Yokoyama, Naoaki; Suzuki, Hiroshi; Igarashi, Ikuo; Maeda, Ryuichiro; Inpankaew, Tawin; Jittapalapong, Sathaporn; Xuan, Xuenan (2012)Although Babesia bovis and Babesia bigemina infections cause economic losses in the cattle industry in northern Thailand, there is inadequate information on Babesia isolates present in the area. Therefore, to determine the ... -
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
Yu, Longzheng; Yamagishi, Junya; Zhang, Shoufa; Jin, Chunmei; Aboge, Gabriel Oluga; Zhang, Houshuang; Zhang, Guohong; Tanaka, Tetsuya; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan (2012)A heterologous prime-boost strategy with priming plasmid DNA followed by recombinant virus expressing relevant antigens is known to stimulate protective immunity against intracellular parasites. In this study, we have ... -
Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method
Zhang, Houshuang; Thekisoe, Oriel M.M; Aboge, Gabriel O; Kyan, Hisako; Yamagishi, Junya; Inoue, Noboru; Nishikawa, Yoshifumi; Zakimi, Satoshi; Xuan, Xuenan (2009)Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high specificity, sensitivity and rapidity. In this study, we used a conserved sequence in the 200- to 300-fold repetitive 529 bp gene of Toxoplasma ...