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    Constituents of the Roots and Leaves of Ekebergia capensis and Their Potential Antiplasmodial and Cytotoxic Activities

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    Date
    2014
    Author
    Yenesew, Abiy
    Erdélyi, Máté
    Midiwo, Jacob
    Kimani, Francis
    Landberg, Göran
    Fitzpatrick, Paul A
    Gruhonjic, Amra
    Orwa, Jennifer A
    Irungu, Beatrice N
    Type
    Article; en
    Language
    en
    Metadata
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    Abstract
    A new triterpenoid, 3-oxo-12β-hydroxy-oleanan-28,13β-olide (1), and six known triterpenoids 2–7 were isolated from the root bark of Ekebergia capensis, an African medicinal plant. A limonoid 8 and two glycoflavonoids 9–10 were found in its leaves. The metabolites were identified by NMR and MS analyses, and their cytotoxicity was evaluated against the mammalian African monkey kidney (vero), mouse breast cancer (4T1), human larynx carcinoma (HEp2) and human breast cancer (MDA-MB-231) cell lines. Out of the isolates, oleanonic acid (2) showed the highest cytotoxicity, i.e., IC50’s of 1.4 and 13.3 µM against the HEp2 and 4T1 cells, respectively. Motivated by the higher cytotoxicity of the crude bark extract as compared to the isolates, the interactions of oleanonic acid (2) with five triterpenoids 3–7 were evaluated on vero cells. In an antiplasmodial assay, seven of the metabolites were observed to possess moderate activity against the D6 and W2 strains of P. falciparum (IC50 27.1–97.1 µM), however with a low selectivity index (IC50(vero)/IC50(P. falciparum-D6) < 10). The observed moderate antiplasmodial activity may be due to general cytotoxicity of the isolated triterpenoids. Keywords: Ekebergia capensis; tritepenoid; antiplasmodial; cytotoxicity; Vero; 4T1; HEp2; MDA-MB-231; 3-oxo-12β-hydroxy-oleanan-28,13β-olide
    URI
    http://www.mdpi.com/1420-3049/19/9/14235/htm
    http://hdl.handle.net/11295/77872
    Citation
    Irungu, Beatrice N., Jennifer A. Orwa, Amra Gruhonjic, Paul A. Fitzpatrick, Göran Landberg, Francis Kimani, Jacob Midiwo, Máté Erdélyi, and Abiy Yenesew. "Constituents of the Roots and Leaves of Ekebergia capensis and Their Potential Antiplasmodial and Cytotoxic Activities." Molecules 19, no. 9 (2014): 14235-14246.
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    • Faculty of Science & Technology (FST) [4264]

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