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dc.contributor.authorYenesew, Abiy
dc.contributor.authorErdélyi, Máté
dc.contributor.authorMidiwo, Jacob
dc.contributor.authorKimani, Francis
dc.contributor.authorLandberg, Göran
dc.contributor.authorFitzpatrick, Paul A
dc.contributor.authorGruhonjic, Amra
dc.contributor.authorOrwa, Jennifer A
dc.contributor.authorIrungu, Beatrice N
dc.identifier.citationIrungu, Beatrice N., Jennifer A. Orwa, Amra Gruhonjic, Paul A. Fitzpatrick, Göran Landberg, Francis Kimani, Jacob Midiwo, Máté Erdélyi, and Abiy Yenesew. "Constituents of the Roots and Leaves of Ekebergia capensis and Their Potential Antiplasmodial and Cytotoxic Activities." Molecules 19, no. 9 (2014): 14235-14246.en_US
dc.description.abstractA new triterpenoid, 3-oxo-12β-hydroxy-oleanan-28,13β-olide (1), and six known triterpenoids 2–7 were isolated from the root bark of Ekebergia capensis, an African medicinal plant. A limonoid 8 and two glycoflavonoids 9–10 were found in its leaves. The metabolites were identified by NMR and MS analyses, and their cytotoxicity was evaluated against the mammalian African monkey kidney (vero), mouse breast cancer (4T1), human larynx carcinoma (HEp2) and human breast cancer (MDA-MB-231) cell lines. Out of the isolates, oleanonic acid (2) showed the highest cytotoxicity, i.e., IC50’s of 1.4 and 13.3 µM against the HEp2 and 4T1 cells, respectively. Motivated by the higher cytotoxicity of the crude bark extract as compared to the isolates, the interactions of oleanonic acid (2) with five triterpenoids 3–7 were evaluated on vero cells. In an antiplasmodial assay, seven of the metabolites were observed to possess moderate activity against the D6 and W2 strains of P. falciparum (IC50 27.1–97.1 µM), however with a low selectivity index (IC50(vero)/IC50(P. falciparum-D6) < 10). The observed moderate antiplasmodial activity may be due to general cytotoxicity of the isolated triterpenoids. Keywords: Ekebergia capensis; tritepenoid; antiplasmodial; cytotoxicity; Vero; 4T1; HEp2; MDA-MB-231; 3-oxo-12β-hydroxy-oleanan-28,13β-olideen_US
dc.titleConstituents of the Roots and Leaves of Ekebergia capensis and Their Potential Antiplasmodial and Cytotoxic Activitiesen_US

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