Enrichment of LAG-3, but not PD-1, on Double Negative T Cells at the Female Genital Tract
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Date
2014-12Author
Juno, Jennifer A
Lajoie, Julie
Stalker, Andrew T
Oyugi, Julius
Kimani, Makobu
Kimani, Joshua
Plummer, Francis A
Fowke, Keith R
Language
enMetadata
Show full item recordAbstract
Problem
The expression of inhibitory markers such as LAG-3 and PD-1 on T lymphocytes regulates immune function. Their expression at the genital mucosa is poorly understood, but regulation of immune activation at the female genital tract likely controls susceptibility to sexually transmitted infections.
Method of study
Cervical mononuclear cells were phenotyped by flow cytometry. Concentrations of cytokines were determined in cervical-vaginal lavage samples by bead array.
Results
LAG-3 expression was significantly elevated at the genital mucosa and was associated with expression of CCR5 and CD69. Double negative (DN) T cells expressed the highest levels of LAG-3, but not PD-1, and were more activated than other T lymphocytes.
Conclusion
The elevated expression of LAG-3 at the genital tract suggests it may regulate T-cell activation, and identify cells susceptible to HIV infection. The enrichment of LAG-3 on DN T cells suggests LAG-3 may contribute to the immunoregulatory activity of these cells.
URI
http://onlinelibrary.wiley.com/doi/10.1111/aji.12308/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=falsehttp://hdl.handle.net/11295/78591
Citation
American Journal of Reproductive Immunology Volume 72, Issue 6, pages 534–540, December 2014Publisher
University of Nairobi
Collections
- Faculty of Health Sciences (FHS) [10377]